Silkworms were challenged with Gram-negative Escherchai coli and Gram-positive Bacillus bombyseptieus, respectively. Protein expressions of hemolymph, midgut and integument were determined using two-dimensional gel electrophoresis and miatrix-assisted laser desorption ionization time of flight mass spectrometry 24 hpi. Totally, 47 differentially expressed proteins were identified. 21 up-regulated and 26 down-regulated proteins were determined in E. coli infected silkworm. 15 up-regulated and 30 down-regulated including 3 lost proteins were identified in B. bombyseptieus challenged silkworms .
Two-dimensional gel electrophoresis maps of proteins from challengend silkworm tissues. ‘h’ - hemolymph (A–C), ‘m’ - midgut (D–F), ‘i’ - integument (G–I). Proteins from control silkworms (B, E and H), E. coli infected silkworms (A, D and G) and B. bombyseptieus infected silkworms (C, F and I) reared on artificial diet. Circles and ellipses indicate the successfully identified protein spots .
- Xiao-Wu Zhong, Ping Zhao, Yong Zou, et al. Proteomic analysis of the immune response of the silkworm infected by Escherichia coli and Bacillus bombyseptieus. Insect Science. 2012, 19, 559–569, DOI 10.1111/j.1744-7917.2011.01479.x.