SilkPathDB BLAST is a set of tools built with different BLAST softwares, including NCBI-BLAST+, NCBI-BLAST, AB-BLAST and BLAST 2 SEQUENCES, and used for aligning and searching homologous sequences in the database. It supports aligning aginst multiple databases and exporting matched sequences and regions easily. To search homologous sequences in the database, we strongly recommend the latest NCBI-BLAST+.
The NCBI-BLAST+ is buit with the latest version of NCBI BLAST+, ncbi-blast-2.7.1+-x64-linux. It supports searching against multiple databases in one run, which is the major difference compared with the NCBI-BLAST and AB-BLAST. The NCBI BLAST+ is an optimized version of the former NCBI BLAST, which is not supported by the National Center for Biotechnology Information (NCBI).
The BLAST 2 SEQUENCES is buit with bl2seq program in the NCBI BLAST package, which can be used to compare two sequences.