SilkPathDB BLAST is a set of tools built with NCBI-BLAST+, and used for aligning and searching homologous sequences in the database. It supports aligning aginst multiple databases and exporting matched sequences and regions.
The NCBI-BLAST+ is buit with the latest version of NCBI BLAST+, ncbi-blast-2.8.1+-x64-linux. It supports searching against multiple databases in one run, which is the major difference compared with the NCBI-BLAST and AB-BLAST. The NCBI BLAST+ is an optimized version of the former NCBI BLAST, which is not supported by the National Center for Biotechnology Information (NCBI).
The BLAST 2 SEQUENCES is buit with bl2seq program in the NCBI BLAST package, which can be used to compare two sequences.