Bacillus bombysepticus Wang

Category
Bacteria
Phylum
Firmicutes
Class
Bacilli
Order
Bacillales
Family
Bacillaceae
Genus
Bacillus
Species
bombysepticus
Strain
Wang
Host
Silkworm
Status
Complete
Source
NCBI

Bacillus bombysepticus was first separated from the sick silkworm larvae cadavers and identified by Hartman in 1931 (Hartman 1931). B. bombysepticus is a Gram-positive bacterium and can produce spores and parasporal crystals. B. bombysepticus can infect silkworm and will result in a typical symptom of a disease: a peutz first appears on its thoracoabdominal region or the first 1–3 abdominal aspect, and then expands to the whole body (Huang et al., 2009).

Phylogenetic tree of 16S rRNA sequences and survival curve of Bb infection the silkworm (Huang et al., 2009)

(a) Phylogenetic tree with the sequences of 16S rRNA gene of Bb and its related species. Bb can produce spores and parasporal crystal. (b) Survival curve of Bb oral infection silkworm on day 3 of the fifth instar. Control 1: ddH2O feed silkworm rearing under the condition of temperature of 30°C and humidity of 90%. Control 2: ddH2O feed silkworm rearing under the condition of temperature of 25°C and humidity of 70%. Induced 1: Bb feed silkworm rearing under the condition of temperature of 30°C and humidity of 90%. Induced 2: Bb feed silkworm rearing under the condition of temperature of 25°C and humidity of 70%. (c) Natural infection and oral infection both can cause silkworm cuticle peutz.

The genome of B. bombysepticus str. Wang was sequenced and published by Dr. Cheng et al using high-throughput Solexa paired-end sequencing technology at the Beijing Genomics Institute (BGI) (Shenzhen, China). Totally, 5.8 Mb sequences were assembled, including a circular chromosome (5,295,783 bp) and a circular plasmid (577,809 bp) named pBb. 5,702 genes containing 5,488 protein-coding genes were predicted (Cheng et al, 2014). All sequences were deposited at GenBank under accession no. CP007512 (chromosome) and CP007513 (plasmid pBb).

Data summary of B. bombysepticus Wang

Data TypeRecordsLength (bp or aa)
Assembly25873592
Gene56214830848
Pseudogene5040756
mRNA54874763367
Protein54871582302
rRNA3959813
tRNA947262
ncRNA1406
Exon56214836469

References:

  • Hartman E. A flacherie disease of silkworm caused by Bacillus bombysepticus. Lignan Science Journal. 1931, 10: 279–289.
  • Huang L., Cheng T., Xu P., Cheng D., Fang T., Xia Q. A genome-wide survey for host response of silkworm, Bombyx mori during pathogen Bacillus bombysepticus infection. PLoS ONE. 2009, 4:E8098.
  • Cheng,T., Lin,P., Jin,S., Wu,Y., Fu,B., Long,R., Liu,D., Guo,Y., Peng,L. and Xia,Q. Complete Genome Sequence of Bacillus bombysepticus, a Pathogen Leading to Bombyx mori Black Chest Septicemia. Genome Announc. 2014, 2(3). pii: e00312-14. doi: 10.1128/genomeA.00312-14.